Sample Prep:Article Title: CRISPR screens and lectin microarrays identify high mannose N-glycan regulators
Article Snippet: .. Methodology Sample preparation Instrument Software mouse anti-GM130 (1:250), BD Biosciences 610822 rabbit anti-TGN46 (1:500), Proteintech 13573-1-AP rabbit anti-CCDC22 (1:500), Proteintech 16636-1-AP mouse anti-GM130 (1:250), BD Biosciences 610822 validation: P. Marra et al., "The GM130 and GRASP65 Golgi proteins cycle through and define a subdomain of the intermediate compartment", Nat Cell Biol 2001 rabbit anti-TGN46 (1:500), Proteintech 13573-1-AP validation: X. Wei et al,., "Host RAB11FIP5 protein inhibits the release of Kaposi’s sarcoma-associated herpesvirus particles by promoting lysosomal degradation of ORF45", Plos Pathogens 2020 rabbit anti-CCDC22 (1:500), Proteintech 16636-1-AP validation: KE. .. McNally et al., "Retriever is a multiprotein complex for retromerindependent endosomal cargo recycling", Nat Cell Biol 2017 Cell lines used: A549 (ATCC CCL-185), obtained from UC Berkeley Cell Culture Facility K562 (ATCC CCL-243), obtained from UC Berkeley Cell Culture Facility Jurkat(ATCC TIB-152), obtained from UC Berkeley Cell Culture Facility UC Berkeley Cell Culture Facility obtained the cell lines directly from ATCC and performed authentication then.
Software:Article Title: CRISPR screens and lectin microarrays identify high mannose N-glycan regulators
Article Snippet: .. Methodology Sample preparation Instrument Software mouse anti-GM130 (1:250), BD Biosciences 610822 rabbit anti-TGN46 (1:500), Proteintech 13573-1-AP rabbit anti-CCDC22 (1:500), Proteintech 16636-1-AP mouse anti-GM130 (1:250), BD Biosciences 610822 validation: P. Marra et al., "The GM130 and GRASP65 Golgi proteins cycle through and define a subdomain of the intermediate compartment", Nat Cell Biol 2001 rabbit anti-TGN46 (1:500), Proteintech 13573-1-AP validation: X. Wei et al,., "Host RAB11FIP5 protein inhibits the release of Kaposi’s sarcoma-associated herpesvirus particles by promoting lysosomal degradation of ORF45", Plos Pathogens 2020 rabbit anti-CCDC22 (1:500), Proteintech 16636-1-AP validation: KE. .. McNally et al., "Retriever is a multiprotein complex for retromerindependent endosomal cargo recycling", Nat Cell Biol 2017 Cell lines used: A549 (ATCC CCL-185), obtained from UC Berkeley Cell Culture Facility K562 (ATCC CCL-243), obtained from UC Berkeley Cell Culture Facility Jurkat(ATCC TIB-152), obtained from UC Berkeley Cell Culture Facility UC Berkeley Cell Culture Facility obtained the cell lines directly from ATCC and performed authentication then.
Biomarker Discovery:Article Title: CRISPR screens and lectin microarrays identify high mannose N-glycan regulators
Article Snippet: .. Methodology Sample preparation Instrument Software mouse anti-GM130 (1:250), BD Biosciences 610822 rabbit anti-TGN46 (1:500), Proteintech 13573-1-AP rabbit anti-CCDC22 (1:500), Proteintech 16636-1-AP mouse anti-GM130 (1:250), BD Biosciences 610822 validation: P. Marra et al., "The GM130 and GRASP65 Golgi proteins cycle through and define a subdomain of the intermediate compartment", Nat Cell Biol 2001 rabbit anti-TGN46 (1:500), Proteintech 13573-1-AP validation: X. Wei et al,., "Host RAB11FIP5 protein inhibits the release of Kaposi’s sarcoma-associated herpesvirus particles by promoting lysosomal degradation of ORF45", Plos Pathogens 2020 rabbit anti-CCDC22 (1:500), Proteintech 16636-1-AP validation: KE. .. McNally et al., "Retriever is a multiprotein complex for retromerindependent endosomal cargo recycling", Nat Cell Biol 2017 Cell lines used: A549 (ATCC CCL-185), obtained from UC Berkeley Cell Culture Facility K562 (ATCC CCL-243), obtained from UC Berkeley Cell Culture Facility Jurkat(ATCC TIB-152), obtained from UC Berkeley Cell Culture Facility UC Berkeley Cell Culture Facility obtained the cell lines directly from ATCC and performed authentication then.
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